Dept. of liquid phase separations

Publications since 2000:

Horka M., Kubicek O., Ruzicka F., Hola V., Malinovska I., Slais K.: K., Capillary isoelectric focusing of the native and inactivated microorganisms J. Chromathogr. A, accepted.
Horka M., Ruzicka F., Hola V., Slais K.: Capillary electrophoretic separation of proteins and yeasts dynamically modified by poly(ethylene glycol) pyrenebutanoate with fluorescence detection Electrophoresis, accepted.
Mazanec K., Slais K., Chmelik J.: Mass spectrometric characterization of low-molecular-mass color pI markers and their use for direct determination of pI value of proteins. J. Mass. Spec. 41 (2006), 1570–1577.
Kostal V., Zeisbergerova M., Hrotekova Z., Slais K., Kahle V.: Miniaturized liquid core waveguide-based fluorimetric detection cell for capillary separation methods: Application in CE of amino acids. Electrophoresis 27 (2006), 4658–4665.
Beran M., Prihoda J., Zak Z., Cernik M.: A new route to the syntheses of alkali metal bis(fluorosulfuryl)imides: Crystal structure of LiN(SO2F)(2). Polyhedron 25 (2006), 1292–1298.
Horka M., Ruzicka F., Horky J., Hola V., Slais K.: Capillary isoelectric focusing and fluorometric detection of proteins and microorganisms dynamically modified by poly(ethylene glycol) pyrenebutanoate. Anal. Chem. 24 (2006), 8438–8444.
Zeisbergerova M., Kostal V., Sramkova M., Babica P., Blaha L., Glatz Z., Kahle V.: Separation of microcystins by capillary electrochromatography in monolithic columns. J. Chromatogr. B 841 (2006), 140–144.
Horka M., Ruzicka F., Horky J., Hola V., Slais K.: Capillary isoelectric focusing of proteins and microorganisms in dynamically modified fused silica with UV detection. J. Chromatogr. B 841 (2006), 152–159.
Horka M., Ruzicka F., Hola V., Slais K.: Capillary isoelectric focusing of microorganisms in the pH range 2-5 in a dynamically modified FS capillary with UV detection. Anal. Bioanal. Chem. 385 (2006), 840-846.
Siskova Z., Glatz Z., Kahle V.: Preliminary study on the monitoring of glutathione S-tranferase activity toward styrene oxide by electromigration methods. J. Sep. Sci. 28 (2005), 1357–1362.
Stastna M., Slais K.: Two-dimensional gel isoelectric focusing. Electrophoresis 26 (2005), 3586–3591.
Kostal V., Zeisbergerova M., Slais K., Kahle V.: Fluorescence detection system for capillary separations utilizing a liquid core waveguide with an optical fibre-coupled compact spectrometer. J. Chromatogr. A 1081 (2005) 36-41.
Subert J., Slais K.: Advances in enantioselective TLC. Ces. slov. Farm. 54 (2005), 159-162.
Horka M., Ruzicka F., Hola V., Slais K.: Dynamic modification of microorganisms by pyrenebutanoate for fluorometric detection in capillary zone electrophoresis. Electrophoresis 26 (2005), 548-555.
Stastna M., Slais K.: Colored pI standards and gel isoelectric focusing in strongly acidic pH. Anal. Bioanal. Chem. 382 (2005), 65-72.
Stastna M., Travnicek M., Slais K.: New azo dyes as colored isoelectric point markers for isoelectric focusing in acidic pH region. Electrophoresis 26 (2005), 53-59.
Kahle V., Kostal V., Zeisbergerova M.: Sample injection in capillary electrochromatography by heart-cut technique. J. Chromatogr. A 1044 (2004) 259-265.
Stastna M., Slais K.: Dynamics of isoelectric focusing with ampholitic dyes monitored by camera in real-time. J. Chromatogr. A 1008 (2003) 193-203.
Horka M., Planeta J., Ruzicka F., Slais K.: Sol-gel column technology for capillary isoelectric focusing of microorganisms and biopolymers with UV and fluorimetric detection. Electrophoresis 24 (2003) 1383-1390.
Kahle V., Vazlerova M., Welsch T.: Automated microgradient system for capillary electrochromatography. J. Chromatogr. A 1-2 (2003) 3-9.
Slais K., Horka M., Novackova J., et al.: Fluorescein-based pl markers for capillary isoelectric focusing with laser-induced fluorescence detection. Electrophoresis 11 (2002), 1682-1688.
Cretich M., Stastna M., Chrambach A.: Decreased protein peak asymmetry and width due to static capillary coating with hydrophilic derivatives of polydimethyl-
-acrylamide. Electrophoresis 14 2274-2278 (2002).
Horka M., Slais K.:Pyrenebutanoate as a dynamic protein modifier for fluorometric detection in capillary zone electrophoresis. Electrophoresis 7-8 (2002), 1090-1095.
Radko S.P., Stastna M., Chrambach A.: Polydispersity of liposome preparations as a likely source of peak width in capillary zone electrophoresis. J. Chromatogr. B 761 (2001) 69-75 .
Buzas Z., Chang H.T., Vieira N.E., Yergey A.L., Stastna M., Chrambach A.:
Direct vertical electroelution of protein from a PhastSystem band for mass spectrometric identification at the level of a few picomoles. Proteomics 5 (2001),
691-698.
Subert J., Slais K.: Progress in the separation of enantiomers of chiral drugs by TLC without their prior derivatization. Pharmazie 5 (2001), 355-360.
Kvasnickova L., Glatz Z., Stìrbova H., Kahle V., Slanina J., Musil P.: Application of capillary electrochromatography using macroporous polyacrylamide columns for the analysis of lignans from seeds of Schisandra chinensis. J. Chromatogr. A 916 (2001), 265-271..
Stastna M., Radko S.P., Chrambach A.: Discrimination between peak spreading in capillary zone electrophoresis of proteins due to interaction with the capillary wall and due to protein microheterogeneity. Electrophoresis 22 (2001), 66-70.
Horka M., Willimann T., Blum M., Nording P., Friedl Z., Slais K.: Capillary IEF with UV induced fluorescence detection. J. Chromatogr. A 916 (2001), 65-71.
Radko S.P., Stastna M., Chrambach A.: Size-dependent electrophoretic migration and separation of liposomes by capillary zone electrophoresis in electrolyte solutions of various ionic strengths. Anal. Chem. 24 (2000), 5955-5960.
Radko S.P., Stastna M., Chrambach A.: Capillary zone electrophoresis of sub-mu m-sized particles in electrolyte solutions of various ionic strengths: Size-dependent electrophoretic migration and separation efficiency. Electrophoresis, 17 (2000) 3583-3592.
Que A.H., Kahle V., Novotny M.V.: A microgradient elution system for capillary electrochromatography. J. Microcolumn Sep. 1 (2000), 1-5.