Department of liquid phase separations

Staff

• Doc. RNDr. Karel Šlais, DrSc.  (+420) 532 290 211
• RNDr. Vladislav Kahle, CSc.  (+420) 532 290 222
• RNDr. Miroslava Šťastná, Ph.D.  (+420) 532 290 217
• Ing. Marie Horká, CSc.  (+420) 532 290 221
• Ing. Dana Moravcová, Ph.D.  (+420) 532 290 218
• Olga Smolová  (+420) 532 290 212

Students

• Ing. Anna Kubesová  (+420) 532 290 220
• Mgr. Marie Vykydalová  (+420) 532 290 220

Former members

• RNDr. Martin Beran Ph.D.
• Ing. Vratislav Košťál, Ph.D.

Exploring the world of

Theory, methodology and development of miniaturized analytical separation methods including microcolumn liquid chromatography, capillary liquid chromatography, capillary electrophoresis and electrochromatography and 2D electrophoresis. Main topics involve miniaturization of instrumentation including column technology, detection (photometric, fluorometric, mass spectrometry), electrolyte optimization and development of standards for electrophoresis and isoelectric focusing.

Projects

Current projects

• Divergent flow IEF
• IEF of low-molecular-mass colored pI markers
• IEF in acidic pH
• Dynamic modification of proteins and microorgnisms in capillary electromigration separations with UV and fluorometric detection
• Laser induced fluorescence detection utilizing liquid core waveguides
• Automatization of electroseparation methods, especially CE, CEC and CIEF.
• Column technology for CEC (packed, monolithic, sol-gel).
• Fused silica coating modifications for electrophoretic separations of unicellular organisms.
• Detection for electroseparation methods (LIF, MS).

Current grants

• Rapid detection and identification of pathogenic microorganisms and viruses by electromigration techniques and mass spectrometry.
  IAAX00310701 (2007-2011) Grant Agency of the Academy of Sciences of the Czech Republic
  Principal investigator: Ing. Marie Horká, CSc.
• Integrated bioanalytical technologies for microanalyses and diagnostics with laser induced fluorescence and mass spectrometry coupling.
  LC06023 (2006-2010) Ministry of Education, Youth and Physical Training of the Czech Republic
  Co-investigator: Ing. František Foret, CSc.
• Biofilm - významný faktor virulence u infekcí močových katétrů; detekce a možnost ovlivnění
  NS9678-4 (2008-2011) Ministry of Health of the Czech Republic
  Co-investigator: Ing. Marie Horká, CSc.