Mgr. Michael Killinger, DiS.
Ph.D. student of Analytical Chemistry (MUNI)
Supervisor: Ing. Karel Klepárník, CSc.
I focus on the implementation of modern instrumentation in biological and
clinical research. Currently, I concentrate on the improvement of methodology
for single-cell protein detection and the development of a microfluidic culturing
platform for 3D cell cultures related to hard tissue research and disease
modeling.
Ongoing Projects
Single-cell protein detection
The objective of the project is the development and application of an ultrasensitive and highly selective
bioluminescence method to quantify proteins level heterogeneity in individual cells.
Development of a platform for cell cultures cultivation
In this project, we want to establish a miniaturized platform for generating the 3D cell cultures using
microfabrication (micro - CNC machine), 3D printing, and photolithography. processes such as cell migration,
communication, etc.
Bachelor thesis according to actual collaboration projects.
Available Projects for Students
→ Single-cell Analysis
→ Fluorescence Microscopy
→ Immunocytochemistry
→ Histology
→ 3D Printing
→ Photolithography
→ PDMS Microfluidics
→ 3D Cell Cultures
→ Dynamic Cell Cultivation
Recent Cooperations
Czech Academy of Science (Animal Physiology and Genetics) –Single-cell detection, microfluidic chip
development
Dynamic cultivation
Microfluidic technology allows controlled conditions, automatization, reduced amount of reagents and cells,
and mainly dynamic conditions for continuous perfusion of nutrients and removal of the metabolic waste of
the cells. The flow of media also exposes the cell to shear stress similar to the tissue microenvironment.
Specific microfluidic devices can be designed to mimic the microarchitecture and function of some organs or
diseases. In this project, we want to develop a system mimicking the bone environment (bone on a chip) for
studying physiological and pathological processes.
Department of Bioanalytical Instrumentation
www.iach.cz
Institute of Analytical Chemistry of the CAS
Veveří 967/97, 602 00 Brno, Tel: + 420 522 290 111, E-mail: uiach@iach.cz
Masaryk University, Department of Chemistry –Mapping of elements by ICP-MS
Masaryk University, Department of Pathological Physiology –Modern in vitro methods in orthopedic
